In the era of precision medicine, biomarker identification is pivotal for informed clinical decisions. RNA sequencing (RNASeq) has revolutionized this field, offering comprehensive gene expression profiling. This session compares RNASeq with other multiplex gene profiling technologies, assessing factors like sensitivity and scalability. Through case studies, RNASeq's versatility across diverse clinical contexts is illustrated. By providing critical insights, researchers are empowered to leverage these technologies effectively in advancing precision medicine. This exploration highlights RNASeq's transformative potential in unraveling biological complexity and guiding personalized therapeutic interventions.
Learning Objectives:
Understand the fundamental principles of RNA sequencing (RNASeq) and its role in comprehensive gene expression profiling for biomarker discovery in precision medicine.
Compare and contrast RNASeq with alternative multiplex gene profiling technologies, including NanoString nCounter® and qPCR, by evaluating key factors such as sensitivity, specificity, dynamic range, and scalability.
Assess the strengths and limitations of RNASeq and alternative technologies in elucidating biomarker landscapes, empowering researchers and clinicians to make informed choices in selecting the most appropriate platform for their specific research or clinical needs.
